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第十八届国际生物学奥林匹克竞赛实验试题四

TASK A. Sequence Confirmation of a cDNA (23 marks)
一段cDNA序列的组成(23分)
Objective: To isolate plasmid DNA containing a cDNA of interest and to determine the sequence of the cDNA.
目的：分离一段含有待研究之cDNA的质体DNA，并决定此cDNA的序列

Introduction:
To over-express a gene of interest in a plant or animal you must first isolate the gene of interest in the form of a cDNA. You have done this and in order to amplify this DNA, you have cloned it into the pBluescript SK plasmid vector which you have subsequently used to transform bacteria cells. You must now carry out a quick plasmid preparation to isolate the plasmid and confirm the sequence of your cDNA insert.
介绍：要过度表现植物或动物有待研究的一个基因，你必须先以cDNA的形式分离出这段基因；若你已完成分离，要放大这段DNA并clone在pBluescript SK质体的载体中，接着用这载体来转形细菌；你必须快速进行质体制备，以分离质体并确定此cDNA插入段的序列。










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